Figure 3. Overexpressing circARF3 alleviated SAH-induced BBB injury and MyD88-NF-κB pathway-mediated inflammation. (A) The ipsilateral basal cortex was used for histopathological examination. (B, C) The integrity of BBB was detected by HE staining and Evans blue staining. (D) The expression of ZO-1, Occludin, and Claudin-5 in BMECs was examined by WB. (E) IHC was applied to verify microglial/macrophages activation (labeled by Iba1). (F) The MyD88-NF-êB pathway activation was examined by WB. (G) The expression of IL-1â and TNF-á in the brain lesions was monitored by ELISA. NS, *, ***P>0. 05, P<0.05, P<0.001 vs. Sham group. &, &&&P<0.05, P<0.001 vs. SAH group. N=6.