Figure 4. CircARF3 competitively sponged miR-31-5p. (A) The miR-31-5p expression in rat brain tissues was verified by RT-PCR, NS P>0.05 vs. Blank group, *** P<0.001 vs. Vector group. &&& P<0.001 vs. SAH group. (B) Through the Starbase, we also discovered that miR-31-5p bound to circARF3, and the binding sites between them were shown. (C) Dual-luciferase reporter gene assay was adopted to determine the binding relationship between miR-31-5p and circARF3, NSP>0.05, ** P<0.001 vs. miR-NC group. (D) The FISH experiment revealed that circARF3 bound to miR-31-5p in the cytoplasm. (E) CircARF3 overexpression plasmids and miR-31-5p mimics were transfected into BMECs, respectively, and the expression of miR-31-5p in BMECs was determined by RT-PCR. ** P<0.01 vs. Con group, & P<0.05, && P<0.01 vs. OGD/R group; #P<0.05 vs.OGD/R+miR-31-5p. N=3.