Research Paper Volume 13, Issue 17 pp 21345—21363

Up-regulation of circARF3 reduces blood-brain barrier damage in rat subarachnoid hemorrhage model via miR-31-5p/MyD88/NF-κB axis

CircARF3 attenuated OGD/R-mediated integrity destruction of BMECs by sponging miR-31-5p. Primary BMECs were transfected with miR-31-5p mimics and/or circARF3 overexpression plasmids and then treated with OGD/R for 4 hours. (A) The activity of BMEC was detected by the CCK8 experiment. (B) The integrity of BMECs was evaluated by the permeability test. (C) The levels of ZO-1, Occludin and Claudin-5 were compared by WB. *, **PPPP

Figure 5. CircARF3 attenuated OGD/R-mediated integrity destruction of BMECs by sponging miR-31-5p. Primary BMECs were transfected with miR-31-5p mimics and/or circARF3 overexpression plasmids and then treated with OGD/R for 4 hours. (A) The activity of BMEC was detected by the CCK8 experiment. (B) The integrity of BMECs was evaluated by the permeability test. (C) The levels of ZO-1, Occludin and Claudin-5 were compared by WB. *, **P<0.05, P<0.01 vs. OGD/R group. &, &&, &&&P<0.05, P<0.01, P<0.001 vs. OGD/R+miR-31-5p group. N=3.