Figure 3. Effects of DFO on the expression of senescence-related proteins and angiogenic factors. (A) EPCs were treated with the indicated concentration (μM) of DFO for four days. Whole-cell lysates were harvested for electrophoresis, and Western blotting was performed with the indicated antibodies. (B) Quantification of relative senescence-related protein levels, normalized to GAPDH. Values are the mean ± standard deviation of triplicate assays from three independent experiments. ** P < 0.01 compared with the untreated group (0 μM). (C–F) Images are cells treated without (CTRL) and with DFO on the expression of VEGF, Flt-1, KDR and TIE2, respectively. Young EPCs were treated with 3 μM DFO for four days, and then were harvested for staining with the indicated antibodies. Scale bar, 50 μm.