Research Paper Volume 13, Issue 18 pp 22134—22147

Rutin inhibited the advanced glycation end products-stimulated inflammatory response and extra-cellular matrix degeneration via targeting TRAF-6 and BCL-2 proteins in mouse model of osteoarthritis

Rutin suppresses AGE mediated induction on the NF-κB pathway proteins. (A) Western blot analysis for expression of NF-κB pathway proteins BCL-2, p65 and IκBα in nuclei of AGEs chondrocytes treated with Rutin. (B) Immunofluorescence along with DAPI staining for studying the nuclei translocation of BCL-2. (C) Relative protein levels of BCL-2 in AGEs-induced chondrocytes treated with Rutin. The results were mean ± SD. **P

Figure 4. Rutin suppresses AGE mediated induction on the NF-κB pathway proteins. (A) Western blot analysis for expression of NF-κB pathway proteins BCL-2, p65 and IκBα in nuclei of AGEs chondrocytes treated with Rutin. (B) Immunofluorescence along with DAPI staining for studying the nuclei translocation of BCL-2. (C) Relative protein levels of BCL-2 in AGEs-induced chondrocytes treated with Rutin. The results were mean ± SD. **P<0.01 compared to control group, ##P<0.01 compared to AGEs alone treated group.