Figure 2. Elevated AHNAK content represses ovarian cancer cell proliferation along with infiltration in vitro. (A) qRT-PCR illustrated that AHNAK was successfully overexpressed. (B) Cell proliferation analysis was performed. Cell numbers were evaluated at 24 h, 48 h, and at 72 h after incubation by Coulter Counter (Beckman Coulter, USA). (C) CCK-8 assay was performed. (D) Transwell assays were performed (left), after which infiltrating cells were quantified by the Image J software (right). (E) Western blotting assessment of the levels of indicated epithelial and mesenchymal markers. Data are presented as the mean ± SD for n=3, *p < 0.05, **p < 0.01.