Research Paper Volume 13, Issue 17 pp 21435—21450

MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells

Overexpression of miR-185-5p inhibits proliferation, migration and invasion of NSCLC cells. The miR-185-5p mimic and NC mimic were transfected into A549 cells and H2170 cells, respectively. (A) The proliferation of A549 and H2170 cells was assessed by CCK-8 assay, and the results showed that miR-185-5p mimic inhibited cell proliferation. (B) The migration of A549 and H2170 cells was detected by Transwell cell migration assay, and the results showed that miR-185-5p mimic inhibited cell migration. (C) The invasion of A549 and H2170 cells was analyzed by Transwell cell invasion assay, and the results showed that miR-185-5p mimic inhibited cell invasion. N=6, * PP

Figure 2. Overexpression of miR-185-5p inhibits proliferation, migration and invasion of NSCLC cells. The miR-185-5p mimic and NC mimic were transfected into A549 cells and H2170 cells, respectively. (A) The proliferation of A549 and H2170 cells was assessed by CCK-8 assay, and the results showed that miR-185-5p mimic inhibited cell proliferation. (B) The migration of A549 and H2170 cells was detected by Transwell cell migration assay, and the results showed that miR-185-5p mimic inhibited cell migration. (C) The invasion of A549 and H2170 cells was analyzed by Transwell cell invasion assay, and the results showed that miR-185-5p mimic inhibited cell invasion. N=6, * P<0.05, ** P<0.01.