Figure 3. MiR-185-5p directly targets 3'UTR of RAB35. (A) StarBase 3.0 (http://starbase.sysu.edu.cn/) was used to predict the target genes of miR-185-5p, and we found that miR-185-5p binds to RAB35 mRNA 3'UTR. (B) The relative luciferase activity was tested with wild-type and mutant-type RAB35, respectively. (C, D) The miR-185-5p mimic and NC mimic were transfected into A549 cells and H2170 cells, respectively. The mRNA and protein expression of RAB35 in A549 and H2170 cells was analyzed by RT-qPCR and Western blotting, and the results showed that miR-185-5p mimic inhibited mRNA and protein expression of RAB35. β-actin was used as an invariant internal control for calculating protein-fold changes. N=6, ** P<0.01.