Research Paper Volume 13, Issue 17 pp 21435—21450

MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells

NSCLC cell-derived exosomes with RAB35 knockdown or miR-185-5p overexpression inhibit NSCLC cell proliferation. (A) A549 cells (H2170 cells) with knockdown of RAB35 or overexpression of miR-185-5p were incubated with exosomes secreted by A549 cells (H2170 cells). CCK-8 assay was used to measure the proliferation of A549 and H2170 cells at day 1, 2, 3, 4, 5 after transfection. (B) RAB35 siRNA was transfected as negative control group and co-cultured with the exosomes secreted by RAB35 downregulated or miR-185-5p overexpression A549 cells and H2170 cells, respectively. The cell proliferation was measure by CCK-8 assay. N=6, ** P

Figure 8. NSCLC cell-derived exosomes with RAB35 knockdown or miR-185-5p overexpression inhibit NSCLC cell proliferation. (A) A549 cells (H2170 cells) with knockdown of RAB35 or overexpression of miR-185-5p were incubated with exosomes secreted by A549 cells (H2170 cells). CCK-8 assay was used to measure the proliferation of A549 and H2170 cells at day 1, 2, 3, 4, 5 after transfection. (B) RAB35 siRNA was transfected as negative control group and co-cultured with the exosomes secreted by RAB35 downregulated or miR-185-5p overexpression A549 cells and H2170 cells, respectively. The cell proliferation was measure by CCK-8 assay. N=6, ** P<0.01.