Research Paper Volume 13, Issue 17 pp 20935—20961

Hyperbaric oxygen therapy alleviates vascular dysfunction and amyloid burden in an Alzheimer’s disease mouse model and in elderly patients

HBOT attenuates arteriolar luminal diameter but not amyloid deposition around arterioles in 6-month old 5XFAD mice. Arterioles were visualized using immunostaining with anti-SMA antibody while vascular amyloid deposition was visualized using anti-Aβ antibody (4G8). (A, F) Representative images of arterioles and Aβ in hippocampal (A) and cortical fields (F) of HBO-treated wt (n=9, upper right panels) and 5XFAD mice (n=10, lower right panels) and control wt (n=9, upper left panels) and 5XFAD mice (n=10, lower left panels) (x40 magnification, scale bar: 50 μm). White arrows show hippocampal and cortical arterioles. (B–E) and (G–J), Quantification of arteriolar luminal diameters (B, G), arteriolar wall thickness (C, H) and percentages of arterioles that stained positive for Aβ (D, I) and Aβ deposition area around arterioles (E, J) in the hippocampal (B–E) and cortical fields (G–J). Two-way ANOVA and post-hoc Fisher LSD tests were performed. Values represent means ± SEM. * P P

Figure 5. HBOT attenuates arteriolar luminal diameter but not amyloid deposition around arterioles in 6-month old 5XFAD mice. Arterioles were visualized using immunostaining with anti-SMA antibody while vascular amyloid deposition was visualized using anti-Aβ antibody (4G8). (A, F) Representative images of arterioles and Aβ in hippocampal (A) and cortical fields (F) of HBO-treated wt (n=9, upper right panels) and 5XFAD mice (n=10, lower right panels) and control wt (n=9, upper left panels) and 5XFAD mice (n=10, lower left panels) (x40 magnification, scale bar: 50 μm). White arrows show hippocampal and cortical arterioles. (BE) and (GJ), Quantification of arteriolar luminal diameters (B, G), arteriolar wall thickness (C, H) and percentages of arterioles that stained positive for Aβ (D, I) and Aβ deposition area around arterioles (E, J) in the hippocampal (BE) and cortical fields (GJ). Two-way ANOVA and post-hoc Fisher LSD tests were performed. Values represent means ± SEM. * P < 0.05, ** P < 0.01.