Figure 6. HBOT reduces hypoxia and HIF1α transcription factor levels in the hippocampal area of 6-month old 5XFAD mice. (A) Uptake of Hypoxyprobe by low oxygen-bearing cells was visualized by immunostaining. Representative images of the presence of hypoxia in the hippocampal field of HBO-treated wt (n=4, right upper panel) and 5XFAD mice (n=4, right lower panel), and control wt (n=5, left upper panel) and 5XFAD mice (n=3, left lower panel); x4 magnification, scale bar: 1000 μm. (B, C) Quantification of the percentage of the CA3 (B) and CA1 (C) areas presenting Hypoxyprobe-related fluorescence. (D) Western blots of HIF-1α from hippocampi extracted from HBO-treated and control 5XFAD mice and wt littermates. (E) Quantification of Western blots in (D), presented as percentage of wt control, normalized to GAPDH levels (n = 4–5/group). Two-way ANOVA and post-hoc Fisher LSD tests were performed. Values represent means ± SEM. *P < 0.05, ** P < 0.01, *** P < 0.001.