Research Paper Volume 13, Issue 17 pp 21470—21482

SCFAs promote intestinal double-negative T cells to regulate the inflammatory response mediated by NLRP3 inflammasome

Effect of SCFAs on the activation of intestinal NLRP3 inflammasome. (A) Detection of mouse intestinal NLRP3 and Caspase-1 expression by IHC (n = 5): The expression of NLRP3 and Caspase-1 was negative in the WT group and weakly positive in the WT-SCFAs group. SCFAs promoted the activation of NLRP3. The expression of NLRP3 and Caspase-1 was weakly positive in the AD group, and was positive in the AD-SCFAs group, which was significantly higher than that in the AD group. (B–C) The expression of NLRP3 inflammasome-related protein in the intestine (x¯ ± s, n = 10): The expression of NLRP3, cleaved-Caspase-1, ASC and Pro-Caspase-1 in the NLRP3 inflammasome was low in the WT group. Comparison with the WT group, *P #P D–E) The protein expression of microglia activation marker (IBA-1 and GFAP) in brain tissue (x¯ ± s, n = 10): Comparison with the WT group, *P #P

Figure 2. Effect of SCFAs on the activation of intestinal NLRP3 inflammasome. (A) Detection of mouse intestinal NLRP3 and Caspase-1 expression by IHC (n = 5): The expression of NLRP3 and Caspase-1 was negative in the WT group and weakly positive in the WT-SCFAs group. SCFAs promoted the activation of NLRP3. The expression of NLRP3 and Caspase-1 was weakly positive in the AD group, and was positive in the AD-SCFAs group, which was significantly higher than that in the AD group. (BC) The expression of NLRP3 inflammasome-related protein in the intestine (x¯ ± s, n = 10): The expression of NLRP3, cleaved-Caspase-1, ASC and Pro-Caspase-1 in the NLRP3 inflammasome was low in the WT group. Comparison with the WT group, *P < 0.05; comparison with the AD group, #P < 0.05. (DE) The protein expression of microglia activation marker (IBA-1 and GFAP) in brain tissue (x¯ ± s, n = 10): Comparison with the WT group, *P < 0.05; comparison with the AD group, #P < 0.05.