Research Paper Volume 13, Issue 17 pp 21470—21482

SCFAs promote intestinal double-negative T cells to regulate the inflammatory response mediated by NLRP3 inflammasome

Effects of Fas-IN and TNFR1-IN on DNTs and NLRP3 inflammasome activation. (A–C) Results of DNTs detection (x¯ ± s, n = 10): The proportion of DNTs in the spleen of AD mice was relatively low. Comparison of the proportion of DNTs in the spleen in (B), and comparison of the proportion of DNTs in the intestine in C. Significant difference between the groups, P D) Expression of intestinal NLRP3 and cleaved-Caspase-1 by IHC (n = 5): SCFAs could activate the expression of NLRP3 and cleaved-Caspase-1.

Figure 3. Effects of Fas-IN and TNFR1-IN on DNTs and NLRP3 inflammasome activation. (AC) Results of DNTs detection (x¯ ± s, n = 10): The proportion of DNTs in the spleen of AD mice was relatively low. Comparison of the proportion of DNTs in the spleen in (B), and comparison of the proportion of DNTs in the intestine in C. Significant difference between the groups, P < 0.05. (D) Expression of intestinal NLRP3 and cleaved-Caspase-1 by IHC (n = 5): SCFAs could activate the expression of NLRP3 and cleaved-Caspase-1.