SCFAs promote intestinal double-negative T cells to regulate the inflammatory response mediated by NLRP3 inflammasome

Shuiliang Ruan; Liping Zhai; Shasha Wu; Caiqun Zhang; Qiaobing Guan

doi:doi:10.18632/aging.203487

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Research Paper Volume 13, Issue 17 pp 21470—21482

SCFAs promote intestinal double-negative T cells to regulate the inflammatory response mediated by NLRP3 inflammasome

Figure 6. DNTs promoted NLRP3 inflammasome activation and inflammatory response in intestinal macrophages. (A–B) Detection of the expression of NLRP3 and Caspase-1 by IF staining (n = 3): NLRP3 and Caspase-1 was not expressed in the Con group, and the expression level was up-regulated after co-culture with DNTs. (C) Effect of DNTs on macrophage activation and the expression of inflammatory factors (x¯ ± s, n = 3): the level of inflammatory factors in the Con group was low. Comparison with Con group, ^*P < 0.05. (D–E) Effect of NLRP3 inflammasome and related protein expression (x¯ ± s, n = 3): DNTs co-culture significantly activated the expression of NLRP3 inflammasome related proteins, comparison with the Con group, ^*P < 0.05.