Research Paper Volume 13, Issue 17 pp 21483—21496

2,5-dimethyl celecoxib induces apoptosis and autophagy via activation of ROS/JNK axis in nasopharyngeal carcinoma cells

DMC inhibited the proliferation of NPC both in vitro and in vivo. (A, B) CNE-2 and CNE-2R cells were incubated with 0-100 μM of DMC or 0.1% DMSO for 24 h -72 h, and cells viability was then quantified by the MTT assay. (C, D) The ability of single CNE-2 and CNE-2R cell to form clones after treating with 0.1% DMSO, 20 μM, or 40 μM DMC assessed by colony formation assay. (E) Representative images of BALB/c-nude xenografts from nude mice following the injections of CNE-2 cells in control or DMC group. (F) Tumor volumes of xenografts in nude mice for each group was recorded every 4 days after DMC treatment. (G) The tumors were removed and the tumor weight for each group was recorded after all mice were killed. (H) Tumor specimens were analyzed by immunohistochemical staining with LC3-II antibodies. **P **P ***P

Figure 1. DMC inhibited the proliferation of NPC both in vitro and in vivo. (A, B) CNE-2 and CNE-2R cells were incubated with 0-100 μM of DMC or 0.1% DMSO for 24 h -72 h, and cells viability was then quantified by the MTT assay. (C, D) The ability of single CNE-2 and CNE-2R cell to form clones after treating with 0.1% DMSO, 20 μM, or 40 μM DMC assessed by colony formation assay. (E) Representative images of BALB/c-nude xenografts from nude mice following the injections of CNE-2 cells in control or DMC group. (F) Tumor volumes of xenografts in nude mice for each group was recorded every 4 days after DMC treatment. (G) The tumors were removed and the tumor weight for each group was recorded after all mice were killed. (H) Tumor specimens were analyzed by immunohistochemical staining with LC3-II antibodies. **P < 0.01, **P < 0.001, and ***P < 0.001, significantly different compared with control group.