Figure 3. DMC induced autophagic flux in NPC cells. (A) Fluorescence photographs of CNE-2 and (B) CNE-2R cells transfected with mRFP-GFP-LC3B reporter. Cells were treated with 0.1% DMSO, 20 μM DMC, 10 μM RAPA, 20 μM CQ or DMC+CQ for 4 h. CQ-treated cells were used as positive controls. Nuclei were stained with DAPI. Scale bar: 10 μm. Average numbers of autophagosomes (yellow puncta) and autolysosomes (red puncta) per cell, and over 30 cells were counted in each condition. (n = 3; **P < 0.01; ***P < 0.001 compared with control group).