Research Paper Volume 13, Issue 17 pp 21497—21512

m6A demethylase ALKBH5 suppression contributes to esophageal squamous cell carcinoma progression

ALKBH5 overexpression suppressed in vitro proliferation and in vivo tumorigenesis of ESCC cells. (A, B) qRT-PCR (A) and western blot (B) analysis of ALKBH5 expression in vector-expressing (LV-con) and ALKBH5-expressing (LV-ALKBH5) ESCC cells (i.e., TE-13, Eca-109, and KYSE-150 cells). (C, D) A colony formation assay was performed to study the proliferation ability of vector- and ALKBH5-expressing ESCC cells. Left panels show representative images of colony formation assay (C) and right panels signify the total colony count (D). (E, F) Effects of ALKBH5 overexpression on cell cycle distribution in TE-13, Eca-109, and KYSE-150 cells. (G–I) ALKBH5 overexpression suppressed tumor growth of Eca-109 cells in nude mice. Vector- or ALKBH5-expressing Eca-109 cells were subcutaneously injected into the left and right dorsal thighs of mice, respectively. (G) Representative image of tumors formed. (H) Growth curve of tumor volumes. (I) Tumors were weighed.

Figure 2. ALKBH5 overexpression suppressed in vitro proliferation and in vivo tumorigenesis of ESCC cells. (A, B) qRT-PCR (A) and western blot (B) analysis of ALKBH5 expression in vector-expressing (LV-con) and ALKBH5-expressing (LV-ALKBH5) ESCC cells (i.e., TE-13, Eca-109, and KYSE-150 cells). (C, D) A colony formation assay was performed to study the proliferation ability of vector- and ALKBH5-expressing ESCC cells. Left panels show representative images of colony formation assay (C) and right panels signify the total colony count (D). (E, F) Effects of ALKBH5 overexpression on cell cycle distribution in TE-13, Eca-109, and KYSE-150 cells. (GI) ALKBH5 overexpression suppressed tumor growth of Eca-109 cells in nude mice. Vector- or ALKBH5-expressing Eca-109 cells were subcutaneously injected into the left and right dorsal thighs of mice, respectively. (G) Representative image of tumors formed. (H) Growth curve of tumor volumes. (I) Tumors were weighed.