Research Paper Volume 13, Issue 17 pp 21497—21512

m6A demethylase ALKBH5 suppression contributes to esophageal squamous cell carcinoma progression

RNAi-induced silencing of endogenous ALKBH5 promoted in vitro proliferation and in vivo tumorigenesis of ESCC cells. (A, B) qRT-PCR (A) and western blot (B) analysis of ALKBH5 expression in shSCR-expressing (LV-shSCR) and shALKBH5-expressing (LV-shALKBH5-1 and LV-shALKBH5-2) ESCC cells (TE-13 and Eca-109 cells). (C, D) A colony formation assay was performed to study the proliferation ability of shSCR- and shALKBH5-expressing ESCC cells. Left panels show representative images of colony formation assay (C) and right panels signify the total colony count (D). (E, F) Effects of RNAi-induced ALKBH5 silencing on cell cycle distribution in TE-13 and Eca-109 cells. (G–I) ALKBH5 knockdown enhanced tumor growth of TE-13 cells in nude mice. shSCR- or shALKBH5-expressing TE-13 cells were subcutaneously injected into the left and right dorsal thighs of mice, respectively. (G) Representative image of tumors formed. (H) Growth curve of tumor volumes. (I) Tumors were weighed.

Figure 3. RNAi-induced silencing of endogenous ALKBH5 promoted in vitro proliferation and in vivo tumorigenesis of ESCC cells. (A, B) qRT-PCR (A) and western blot (B) analysis of ALKBH5 expression in shSCR-expressing (LV-shSCR) and shALKBH5-expressing (LV-shALKBH5-1 and LV-shALKBH5-2) ESCC cells (TE-13 and Eca-109 cells). (C, D) A colony formation assay was performed to study the proliferation ability of shSCR- and shALKBH5-expressing ESCC cells. Left panels show representative images of colony formation assay (C) and right panels signify the total colony count (D). (E, F) Effects of RNAi-induced ALKBH5 silencing on cell cycle distribution in TE-13 and Eca-109 cells. (GI) ALKBH5 knockdown enhanced tumor growth of TE-13 cells in nude mice. shSCR- or shALKBH5-expressing TE-13 cells were subcutaneously injected into the left and right dorsal thighs of mice, respectively. (G) Representative image of tumors formed. (H) Growth curve of tumor volumes. (I) Tumors were weighed.