Figure 4. Ectopic expression of ALKBH5 inhibited the migration and invasion of ESCC cells in vitro. (A–D) Wound healing assays were performed in ALKBH5-expressing ESCC cells. The migration ability was determined by measuring the distance from the boundary of the scratch created to the cell-free space after 24 h. (E, F) The migratory and invasive activities of ALKBH5-expressing ESCC cells based on transwell migration and Boyden invasion assays, respectively. The average number of cells per field was calculated from three independent experiments (original magnification: ×200).