Figure 2. Effects of calories and food quantity on the lifespan and energy state. (A) T1-weighted MRI images of mice, where the highlighted part represents body fat. (B) Body composition of mice in each dietary group at different ages, and the corresponding (C) fat mass as a percentage of total body weight. Representative H&E-stained histological sections of (D) eWAT and (E) BAT under the different feeding conditions, as indicated. (F) Serum leptin concentration (n=6 per group). Data are presented as mean±SEM, #P<0.05, ##P<0.01, ###P<0.001 vs the HF group; *P<0.05, **P<0.01, ***P<0.001 vs the NF group according to ANNOVAS.