Research Paper Volume 13, Issue 17 pp 21526—21546

Calorie intake rather than food quantity consumed is the key factor for the anti-aging effect of calorie restriction

Aging-sensitive markers, physical activity, and glucose tolerance of mice. (A) Locomotor activity of mice at 6, 15, and 24 months of age (n=10 per group). (B) Latency to fall from a rotarod indicated the motor coordination of mice. (C) The results of the Morris water maze test for the mice under different dietary regimens. (D) The recognition index in the novel-object recognition test. (E) Representative immunoblots and densitometric quantification of the immunoblots for phosphor-Ser133-CREB (pCREB) in the mouse liver. (F) Serum glucose concentration in the different experimental groups and the corresponding (G) serum insulin concentrations (n=5 per group). (H) GTTs in the different experimental groups and the corresponding (I) areas under the curve (AUC). Five mice per group were analyzed. Data are presented as mean±SEM, #P##P###P*P**P***P

Figure 3. Aging-sensitive markers, physical activity, and glucose tolerance of mice. (A) Locomotor activity of mice at 6, 15, and 24 months of age (n=10 per group). (B) Latency to fall from a rotarod indicated the motor coordination of mice. (C) The results of the Morris water maze test for the mice under different dietary regimens. (D) The recognition index in the novel-object recognition test. (E) Representative immunoblots and densitometric quantification of the immunoblots for phosphor-Ser133-CREB (pCREB) in the mouse liver. (F) Serum glucose concentration in the different experimental groups and the corresponding (G) serum insulin concentrations (n=5 per group). (H) GTTs in the different experimental groups and the corresponding (I) areas under the curve (AUC). Five mice per group were analyzed. Data are presented as mean±SEM, #P<0.05, ##P<0.01, ###P<0.001 vs the HF group; *P<0.05, **P<0.01, ***P<0.001 vs the NF group according to ANNOVAS.