Figure 5. Quantitative analysis of the expression of proteins related to classic pathways of calorie restriction. (A) Western blotting analysis of SIRT1, SIRT3, SIRT6, phospho-AMPK, Ac-NFĸB in the liver of mice from different experimental groups (n=3 per group). (B) Immunohistochemical analysis of SIRT1 and Ac-NFĸB expression in the liver. #P<0.05, ##P<0.01, ###P<0.001 vs the HF group; *P<0.05, **P<0.01, ***P<0.001 vs the NF group according to ANNOVAS.