Figure 3. MET prevents cardiac remodeling and reduces myocardial fibrosis in aging mice. (A) The expression of cardiac remodeling-associated biomarkers, including ANP and BNP. (B) Quantification of the relative protein expression levels. (C) Sirius Red staining for collagen deposition in the cardiac tissue; (D) Quantification of positive staining. (E) Cardiac collagen III, α-SMA, and TGF-β levels as assessed by western blotting. (F) Quantification of relative protein expression levels. Magnification 40×. The arrows indicate positively stained cells. n = 6–8 per group. *P < 0.05.