Figure 3. HCG11 is a negative regulator on OS growth in vitro and in vivo. (A, B) Overexpression and silence of HCG11 in OS cells validated by qRT-PCR. (C, D) Influence of HCG11 on the proliferation of OS cells was assessed by CCK8 assays. (E, F) Influence of HCG11 on the cell cycle of OS cells was analyzed by flow cytometry. (G, H) Influence of HCG11 on the DNA replication of OS cells was assessed by EdU assay. (I, J) Influence of HCG11 on OS tumor growth in vivo was estimate by Xenograft tumorigenesis. NS, no significance, *P < 0.05, **P < 0.01.