Research Paper Volume 13, Issue 17 pp 21743—21757

lncRNA HCG11 suppresses human osteosarcoma growth through upregulating p27 Kip1

HCG11 raises p27 Kip1 expression via binding to IGF2BP2. (A, B) Regulation of HCG11 and miR-942-5p on p27 Kip1 mRNA level was detected by qRT-PCR. (C) Subcellular localization of IGF2BP2 was confirmed by immunofluorescence. (D, E) Integration of HCG11 and p27 Kip1 mRNA with IGF2BP2 was proved by RIP assay. (F, G) Regulation of HCG11 and IGF2BP2 on p27 Kip1 mRNA and protein level was estimated by qRT-PCR and western blot. (H, I) Influence of HCG11 and IGF2BP2 on p27 Kip1 mRNA degradation was estimated by RNA stability analysis. (J) p27 Kip1 mRNA expression in OS tissues and ANTs was analyzed by qRT-PCR. (K) Correlation between p27 Kip1 mRNA expression and HCG11 expression was analyzed by Spearman’s analysis. *P **P ***P

Figure 5. HCG11 raises p27 Kip1 expression via binding to IGF2BP2. (A, B) Regulation of HCG11 and miR-942-5p on p27 Kip1 mRNA level was detected by qRT-PCR. (C) Subcellular localization of IGF2BP2 was confirmed by immunofluorescence. (D, E) Integration of HCG11 and p27 Kip1 mRNA with IGF2BP2 was proved by RIP assay. (F, G) Regulation of HCG11 and IGF2BP2 on p27 Kip1 mRNA and protein level was estimated by qRT-PCR and western blot. (H, I) Influence of HCG11 and IGF2BP2 on p27 Kip1 mRNA degradation was estimated by RNA stability analysis. (J) p27 Kip1 mRNA expression in OS tissues and ANTs was analyzed by qRT-PCR. (K) Correlation between p27 Kip1 mRNA expression and HCG11 expression was analyzed by Spearman’s analysis. *P < 0.05, **P < 0.01, ***P < 0.001.