Figure 4. Healthspan of combined genetic interventions on odr-3 and ife-2 at 20° C. (A–D) Bar chart representation of motility-assessed healthspan illustrating the fraction of each category upon daily monitorization. Worms are grouped into three categories: mobile (white), impaired (light gray) and frail (dark gray). Dead and censored animals were subtracted from these analyses. (E) Mean number of days in each motility state throughout lifespan. The mean time spent in the impaired state is computed as the difference between the mean time spent as mobile or impaired, and the mean time spent in the mobile state. The mean time spent in the frail state is computed as the difference between the mean lifespan and mean time spent as mobile or impaired. The values within brackets represent the distribution of motion stages during the lifespan. (A–E) WT(EV) and odr-3 denote worms fed with RNAi(EV). (C, D) ife-2 and odr-3; ife-2 denote worms fed with ife-2 RNAi bacteria. (F) The pharyngeal pumping rate (average number of contractions per minute) of WT, odr-3(n1605), ife-2(ok306) and odr-3(n1605); ife-2(ok306) mutants were recorded on days 1, 5, 10 and 15 post-L4 moult. odr-3(n1605); ife-2(ok306) worms show a significantly slower decline of pharyngeal pumping with age, compared to WT. For simplicity, only significant differences among groups are indicated (one way ANOVA with Dunnett's test); ** denotes p < 0.01; *** denotes p < 0.001.