Research Paper Volume 13, Issue 17 pp 21040—21065

Knock-down of odr-3 and ife-2 additively extends lifespan and healthspan in C. elegans

Loss of odr-3 and ife-2 activity enhances oxidative stress tolerance. (A) Survival fraction of the indicated L4 larvae upon 5 days exposure to 0.2M paraquat (the experiment was repeated independently three times). (B) Survival fraction of the indicated strains upon 1 hour treatment with 0.5M NaN3 (the experiment was repeated independently four times). (C) Survival fraction of the indicated strains upon 4h heat stress at 35° C. Each strain was scored on three replicate plates and the experiment was repeated independently four times. (D) Egg-laying rate of the indicated strains. The average number of eggs laid by each strain was determined by transferring worms to new E. coli plates every 12 hours from L4 stage. (E) Brood size of the indicated strains at 20° C. Each point represents the total brood of one hermaphrodite. (A–E) Bars indicate the mean ± SEM. For simplicity, only significant differences among groups are indicated (one way ANOVA with Dunnett's test); * denotes p

Figure 5. Loss of odr-3 and ife-2 activity enhances oxidative stress tolerance. (A) Survival fraction of the indicated L4 larvae upon 5 days exposure to 0.2M paraquat (the experiment was repeated independently three times). (B) Survival fraction of the indicated strains upon 1 hour treatment with 0.5M NaN3 (the experiment was repeated independently four times). (C) Survival fraction of the indicated strains upon 4h heat stress at 35° C. Each strain was scored on three replicate plates and the experiment was repeated independently four times. (D) Egg-laying rate of the indicated strains. The average number of eggs laid by each strain was determined by transferring worms to new E. coli plates every 12 hours from L4 stage. (E) Brood size of the indicated strains at 20° C. Each point represents the total brood of one hermaphrodite. (AE) Bars indicate the mean ± SEM. For simplicity, only significant differences among groups are indicated (one way ANOVA with Dunnett's test); * denotes p < 0.05; ** denotes p < 0.01.