Research Paper Volume 13, Issue 17 pp 21066—21089

Sex-specific differences in DNA double-strand break repair of cycling human lymphocytes during aging

Cell cycle distribution and cell death in PBL from different age groups. Cultivated PBL were fixed and DNA content analyzed by propidium iodide staining and flow cytometry. Percentage of live cells in G1-, S-, and G2-phase (A) and proportion of dead cells, determined by subG1-DNA content (B); female: n=11 (young), n=8 (old); male: n=2 (young), n=6 (old) (Supplementary Table 1).

Figure 2. Cell cycle distribution and cell death in PBL from different age groups. Cultivated PBL were fixed and DNA content analyzed by propidium iodide staining and flow cytometry. Percentage of live cells in G1-, S-, and G2-phase (A) and proportion of dead cells, determined by subG1-DNA content (B); female: n=11 (young), n=8 (old); male: n=2 (young), n=6 (old) (Supplementary Table 1).