Research Paper Volume 13, Issue 18 pp 22176—22187

Key genes affecting the progression of nasopharyngeal carcinoma identified by RNA-sequencing and bioinformatic analysis

Knockdown of CXCL10 inhibited cell proliferation and invasion of NPC cells. (A) The expression of CXCR3 was examined by qRT-PCR assay. The expression of CXCL10 in NPC tumor tissue and normal tissue was examined by IHC analysis. (B) CNE-2 and 5–8F cells were transfected with siNC, siCXCL10-1 or siCXCL10-2, and RT-PCR was performed to examine the CXCL10 expression. (C) Cell proliferation of CNE-2 and 5–8F cells was identified by CCK8 assay. (D) Cell apoptosis of CNE-2 and 5–8F cells was evaluated by FCM. (E) Cell invasion was tested by wound healing assay. (F) Cell migration was detected by Transwell assay. **P

Figure 5. Knockdown of CXCL10 inhibited cell proliferation and invasion of NPC cells. (A) The expression of CXCR3 was examined by qRT-PCR assay. The expression of CXCL10 in NPC tumor tissue and normal tissue was examined by IHC analysis. (B) CNE-2 and 5–8F cells were transfected with siNC, siCXCL10-1 or siCXCL10-2, and RT-PCR was performed to examine the CXCL10 expression. (C) Cell proliferation of CNE-2 and 5–8F cells was identified by CCK8 assay. (D) Cell apoptosis of CNE-2 and 5–8F cells was evaluated by FCM. (E) Cell invasion was tested by wound healing assay. (F) Cell migration was detected by Transwell assay. **P < 0.01 vs siNC group.