Research Paper Volume 13, Issue 17 pp 21778—21790

Transfection of STAT3 overexpression plasmid mediated through recombinant lentivirus promotes differentiation of bone marrow mesenchymal stem cells into neural cells in fetal rats with spina bifida aperta

STAT3 plasmid transfected BMSCs were successfully identified. (A) The expression of CD34, CD45, CD73 and CD90 from BMSCs were detected through the use of flow cytometry. BMSCs proliferation activity at P0 (B), P1-3 (C), P4-6 (D) were determined using the CCK-8 test in the blank control group, negative control group and experimental group prior to transplantation. The STAT3 mRNA expression at P0 (E), P1-3 (F), P4-6 (G). The BMSCs in the experimental group increased more significantly than that in blank control group and negative control group. * P

Figure 1. STAT3 plasmid transfected BMSCs were successfully identified. (A) The expression of CD34, CD45, CD73 and CD90 from BMSCs were detected through the use of flow cytometry. BMSCs proliferation activity at P0 (B), P1-3 (C), P4-6 (D) were determined using the CCK-8 test in the blank control group, negative control group and experimental group prior to transplantation. The STAT3 mRNA expression at P0 (E), P1-3 (F), P4-6 (G). The BMSCs in the experimental group increased more significantly than that in blank control group and negative control group. * P <0.05 versus blank control group + negative control group.