Research Paper Volume 13, Issue 17 pp 21778—21790

Transfection of STAT3 overexpression plasmid mediated through recombinant lentivirus promotes differentiation of bone marrow mesenchymal stem cells into neural cells in fetal rats with spina bifida aperta

BMSCs combined with STAT3 overexpression plasmid transfection can improve the success rate of transplantation. (A) The purity of BMSCs in the experimental group was statistically significant, compared to the blank control group and negative control group. (B) The overall survival rate of BMSCs in the experimental group was found to be slightly higher than that of blank control group and negative control group from E15 to E17, and statistically significant. (C) BMSCs survival was notably increased at P0, P1-3 and P4-6 compared to the blank control group and negative control group (PD) The medium injection group was remarkably different from the change that occurred in the low injection group and high injection group on E19 fetuses after P1-3 BMSCs transplantation, which reached a statistical significance.

Figure 2. BMSCs combined with STAT3 overexpression plasmid transfection can improve the success rate of transplantation. (A) The purity of BMSCs in the experimental group was statistically significant, compared to the blank control group and negative control group. (B) The overall survival rate of BMSCs in the experimental group was found to be slightly higher than that of blank control group and negative control group from E15 to E17, and statistically significant. (C) BMSCs survival was notably increased at P0, P1-3 and P4-6 compared to the blank control group and negative control group (P<0.05). (D) The medium injection group was remarkably different from the change that occurred in the low injection group and high injection group on E19 fetuses after P1-3 BMSCs transplantation, which reached a statistical significance.