Research Paper Volume 13, Issue 17 pp 21778—21790

Transfection of STAT3 overexpression plasmid mediated through recombinant lentivirus promotes differentiation of bone marrow mesenchymal stem cells into neural cells in fetal rats with spina bifida aperta

STAT3 and pSTAT3 expression in the spinal cord of each group after BMSCs transplantation using RT-PCR and western blot analysis. The pSTAT3 mRNA expression was detected utilizing RT-qPCR (A), and protein expression using western blot analysis (B, C). Both pSTAT3 mRNA and protein levels were significantly increased within the experimental group from E18 to E20, with the peak being at E19. In addition, expression of STAT3 mRNA was analyzed by RT-qPCR (D). Protein levels were assessed using western blot (E, F). The expression of STAT3 mRNA and protein levels in the experimental group gradually decreased with embryonic development between E18 and E19; E19 dropped down to bottom. * P

Figure 4. STAT3 and pSTAT3 expression in the spinal cord of each group after BMSCs transplantation using RT-PCR and western blot analysis. The pSTAT3 mRNA expression was detected utilizing RT-qPCR (A), and protein expression using western blot analysis (B, C). Both pSTAT3 mRNA and protein levels were significantly increased within the experimental group from E18 to E20, with the peak being at E19. In addition, expression of STAT3 mRNA was analyzed by RT-qPCR (D). Protein levels were assessed using western blot (E, F). The expression of STAT3 mRNA and protein levels in the experimental group gradually decreased with embryonic development between E18 and E19; E19 dropped down to bottom. * P <0.05 versus blank control group + negative control group.