Figure 5. Nerve markers (GFAP, NSE, NF and nestin) and apoptosis related factors (caspase-8 and bcl-2) expressions were tested using real-time PCR and Western blot. (A) Expression of GFAP, NSE, NF and nestin in the blank control group, negative control group and the experimental group were examined using RT-qPCR. GFAP, NSE, NF and nestin protein expression was analyzed using western blot analysis (B, C). Relative mRNA expression of apoptosis related factors (caspase-8 and bcl-2) was determined using RT-qPCR (D) and protein expression using western blot analysis (E, F). * P < 0.05 versus blank control group + negative control group.