Research Paper Volume 13, Issue 17 pp 21778—21790

Transfection of STAT3 overexpression plasmid mediated through recombinant lentivirus promotes differentiation of bone marrow mesenchymal stem cells into neural cells in fetal rats with spina bifida aperta

Nerve markers (GFAP, NSE, NF and nestin) and apoptosis related factors (caspase-8 and bcl-2) expressions were tested using real-time PCR and Western blot. (A) Expression of GFAP, NSE, NF and nestin in the blank control group, negative control group and the experimental group were examined using RT-qPCR. GFAP, NSE, NF and nestin protein expression was analyzed using western blot analysis (B, C). Relative mRNA expression of apoptosis related factors (caspase-8 and bcl-2) was determined using RT-qPCR (D) and protein expression using western blot analysis (E, F). * P  0.05 versus blank control group + negative control group.

Figure 5. Nerve markers (GFAP, NSE, NF and nestin) and apoptosis related factors (caspase-8 and bcl-2) expressions were tested using real-time PCR and Western blot. (A) Expression of GFAP, NSE, NF and nestin in the blank control group, negative control group and the experimental group were examined using RT-qPCR. GFAP, NSE, NF and nestin protein expression was analyzed using western blot analysis (B, C). Relative mRNA expression of apoptosis related factors (caspase-8 and bcl-2) was determined using RT-qPCR (D) and protein expression using western blot analysis (E, F). * P < 0.05 versus blank control group + negative control group.