Research Paper Volume 13, Issue 18 pp 22188—22207

Cx32 inhibits the autophagic effect of Nur77 in SH-SY5Y cells and rat brain with ischemic stroke

OGD/R induced up-regulation of Cx32 expression and activation of autophagy. (A) Double labeling of Cx32 and β-tubulin in the Ctrl or OGD/R (2h OGD following 24 h recovery) group. Scale bars, 50 μm. (B) The mRNA level of Cx32 was detected by qPCR. (C–D) Representative bands of Cx32 protein in the cells after OGD/R. (E) The autophagosome was labeled with LC3 (green), autophagolysosome was marked by LysoTracker probes (red), and cell nuclei were counterstained by DAPI (blue). Scale bars, 20 μm. (F–G) Representative bands of Beclin1 and LC3 protein in SH-SY5Y cells after OGD/R. Variation in protein loading was determined by blotting with an anti-β-actin antibody. Densitometric scanning of band intensities were calculated as means ± SD (n = 3). *p **p

Figure 1. OGD/R induced up-regulation of Cx32 expression and activation of autophagy. (A) Double labeling of Cx32 and β-tubulin in the Ctrl or OGD/R (2h OGD following 24 h recovery) group. Scale bars, 50 μm. (B) The mRNA level of Cx32 was detected by qPCR. (CD) Representative bands of Cx32 protein in the cells after OGD/R. (E) The autophagosome was labeled with LC3 (green), autophagolysosome was marked by LysoTracker probes (red), and cell nuclei were counterstained by DAPI (blue). Scale bars, 20 μm. (FG) Representative bands of Beclin1 and LC3 protein in SH-SY5Y cells after OGD/R. Variation in protein loading was determined by blotting with an anti-β-actin antibody. Densitometric scanning of band intensities were calculated as means ± SD (n = 3). *p < 0.05 vs. Ctrl group, **p < 0.01 vs. Ctrl group.