Research Paper Volume 13, Issue 18 pp 22188—22207

Cx32 inhibits the autophagic effect of Nur77 in SH-SY5Y cells and rat brain with ischemic stroke

I/R induced up-regulation of Cx32 expression and activation of autophagy. (A) Double labeling of Cx32 and β-tubulin in the brains of Sham or I/R group. Scale bars, 50 μm. (B) Images acquired by transmission electron microscope. The arrow marks autophagic vacuoles. (C–E) Representative bands of autophagy-related proteins after I/R. Variation in protein loading was determined by blotting with an anti-β-actin antibody.

Figure 5. I/R induced up-regulation of Cx32 expression and activation of autophagy. (A) Double labeling of Cx32 and β-tubulin in the brains of Sham or I/R group. Scale bars, 50 μm. (B) Images acquired by transmission electron microscope. The arrow marks autophagic vacuoles. (CE) Representative bands of autophagy-related proteins after I/R. Variation in protein loading was determined by blotting with an anti-β-actin antibody.