Figure 8. 2-APB prohibited the dissociation of Rac1 and Nur77 after I/R injury. (A–B) Representative bands of Rac1 protein in brain tissues after I/R. Statistical results from the densitometric measurements after normalization against β-actin were calculated as means ± SD (n = 3). **p < 0.01 vs. Sham group, ##p < 0.01 vs. I/R. (C) Detect the correlation between Nur77 and Rac1 by a Co-IP assay. (D) Double labeling of Rac1 and Nur77 in the brain tissues of Sham or I/R group. Scale bars, 50 μm.