Research Paper Volume 13, Issue 18 pp 22208—22231

Involvement of c-Myc in low dose radiation-induced senescence enhanced migration and invasion of unirradiated cancer cells

Effects of c-Myc inhibitor (10058-F4) on LDR induced senescence and unirradiated cell migration. (A) Cell survival curve of H1299 cells treated with increased dose of 10058-F4. (B) Luciferase reporter gene assay for demonstrating the effects of 10058-F4 on suppressing c-Myc transcriptional activity in H1299 cells. (C) 10058-F4 suppressed IR (0.5Gy) induced senescence. (D) Quantification of SA-β-gal staining in cells exposed to IR or 10058-F4. (E) Comparison of the effects of CM collected from cells exposed to IR with or without the presence of 10058-F4 (50 μM) on the migration rates of unirradiated cells. (F) Quantification of cell migration by wound healing assay. *p

Figure 5. Effects of c-Myc inhibitor (10058-F4) on LDR induced senescence and unirradiated cell migration. (A) Cell survival curve of H1299 cells treated with increased dose of 10058-F4. (B) Luciferase reporter gene assay for demonstrating the effects of 10058-F4 on suppressing c-Myc transcriptional activity in H1299 cells. (C) 10058-F4 suppressed IR (0.5Gy) induced senescence. (D) Quantification of SA-β-gal staining in cells exposed to IR or 10058-F4. (E) Comparison of the effects of CM collected from cells exposed to IR with or without the presence of 10058-F4 (50 μM) on the migration rates of unirradiated cells. (F) Quantification of cell migration by wound healing assay. *p < 0.05.