Figure 4. Sub-lethal treatment with cisplatin enhances glycolysis in A549 cells. A549 cells were treated with sub-lethal dose of cisplatin and treated with Jarid1b siRNA and metformin. Representative graph of glycolytic cell metabolism of A549 cells analyzed using the Seahorse XFe96 Analyzer (A). A549Res cells increase the extracellular acidification rate (ECAR) after metformin treatment (p<0.05) or Jarid1b inhibition by siRNA (p<0.05) or in the combination on both (p<0.001) (B). Glucose consumption (p<0.001) (C) or lactate production (p<0.001) (D) is increased after sub-lethal treatment with cisplatin for the generation of the A549Res cells. Data represent the mean of four independent experiments.