Research Paper Volume 13, Issue 18 pp 22242—22255

miR-21 regulates ischemic neuronal injury via the p53/Bcl-2/Bax signaling pathway

miR-21/p53 axis regulated Bcl-2/Bax following ischemia in vitro. (A, B) The modulation of p53 mRNA and protein expression by transfecting pcDNA-p53 into primary cultured neurons were confirmed by qRT-PCR (A) and western blot (B). (C, D) Effect of miR-21/p53 axis on the mRNA (C) and protein (D) expression of Bcl-2 and Bax. All experiments were independently repeated three times. *, compared with blank control. *P≤0.05, **P≤0.01. #, compared with miR-21 mimic + vector group. #P≤0.05.

Figure 3. miR-21/p53 axis regulated Bcl-2/Bax following ischemia in vitro. (A, B) The modulation of p53 mRNA and protein expression by transfecting pcDNA-p53 into primary cultured neurons were confirmed by qRT-PCR (A) and western blot (B). (C, D) Effect of miR-21/p53 axis on the mRNA (C) and protein (D) expression of Bcl-2 and Bax. All experiments were independently repeated three times. *, compared with blank control. *P≤0.05, **P≤0.01. #, compared with miR-21 mimic + vector group. #P≤0.05.