Figure 3. Correlation of the two variants and their functional analysis in RNA-seq data of HBV-HCC tumors. (A) The correlation (lower triangle) and frequency distributions (diagonal) of these two variants. The two variants tended to occur simultaneously. (B) Volcano plot for differentially expressed genes between groups with high or low frequency of the two variants. (C) The HBV S gene was significantly upregulated. (D) The gene set represents the gene signature of proliferation and chromosome instability. (E) The gene set represents the gene signature of recurrence-free survival. (F) The gene set represents the gene signature of tumorigenesis. NES, normalized enrichment score. FWER, familywise-error rate.