Research Paper Volume 13, Issue 18 pp 22276—22285

Improvement of cardiac function by mesenchymal stem cells derived extracellular vesicles through targeting miR-497/Smad7 axis

Identification of EVs and measurement of miR-497 in the hypoxia cells. (A) The size distribution of EVs; (B) Morphology of EVs measured by an electron microscope; (C) Specific markers of EVs were measured using western blotting; (D) The mRNA level of miR-497 was measured after different treatments; (E) The levels of EVs and Smad7 were significantly suppressed after hypoxia treatment. + indicated P

Figure 1. Identification of EVs and measurement of miR-497 in the hypoxia cells. (A) The size distribution of EVs; (B) Morphology of EVs measured by an electron microscope; (C) Specific markers of EVs were measured using western blotting; (D) The mRNA level of miR-497 was measured after different treatments; (E) The levels of EVs and Smad7 were significantly suppressed after hypoxia treatment. + indicated P<0.05 compared with the group control. * indicated P<0.05 compared with the group hypoxia. # indicated P<0.05 compared with group hypoxia+EVs.