Research Paper Volume 13, Issue 18 pp 22276—22285

Improvement of cardiac function by mesenchymal stem cells derived extracellular vesicles through targeting miR-497/Smad7 axis

Overexpression of miR-497 reversed the improvement of cardiac function induced by EVs. (A) Influence of EVs and miR-497 mimics on the histological changes of MI animals; (B) Collagen deposition was investigated using Masson trichrome staining; (C) Collagen deposition was investigated using sirius red staining; (D) Influence of EVs and miR-497 mimics on infarction was analyzed; (E) Influence of EVs and miR-497 mimics on left ventricular fractional shortening was analyzed; (F) Influence of EVs and miR-497 mimics on left ventricular ejection fraction was analyzed; (G) Influence of EVs and miR-497 mimics on MDA, SOD, CAT, and GSH-Px was analyzed. + indicated P

Figure 3. Overexpression of miR-497 reversed the improvement of cardiac function induced by EVs. (A) Influence of EVs and miR-497 mimics on the histological changes of MI animals; (B) Collagen deposition was investigated using Masson trichrome staining; (C) Collagen deposition was investigated using sirius red staining; (D) Influence of EVs and miR-497 mimics on infarction was analyzed; (E) Influence of EVs and miR-497 mimics on left ventricular fractional shortening was analyzed; (F) Influence of EVs and miR-497 mimics on left ventricular ejection fraction was analyzed; (G) Influence of EVs and miR-497 mimics on MDA, SOD, CAT, and GSH-Px was analyzed. + indicated P<0.05 compared with the group sham. * indicated P<0.05 compared with the group MI. # indicated P<0.05 compared with group MI+EVs.