Research Paper Volume 13, Issue 18 pp 22332—22344

METTL3-mediated m6A modification of KIF3C-mRNA promotes prostate cancer progression and is negatively regulated by miR-320d

METTL3 facilitated IGF2BP1-regulated stabilization of KIF3C mRNA. (A) RT-qPCR showing METTL3 expression in PCa cell lines. (B) RT-qPCR showing METTL3 knockdown expression in C4–2B and DU145 cells. (C) RIP assay was conducted to show the interaction between METTL3 and KIF3C in PCa cells. (D) m6A RIP assay was conducted to show the m6A modification on KIF3C mRNA in C4–2B and DU145 cells. (E) RT qPCR was conducted to show the stability of KIF3C mRNA after adding ActD. (F) RIP assay was conducted to show the effects of the down-regulation of METTL3 on the interaction between IGF2BP1 and KIF3C in C4–2B and DU145 cells. (G) RT-qPCR showing the stability of KIF3C mRNA after adding ActD in C4–2B and DU145 cells. (H, I) RT-qPCR and western blot were conducted to confirm the KIF3C expression under METTL3 or IGF2BP1 silencing. Data are reported as means ± standard deviation of three independent experiments. *p

Figure 3. METTL3 facilitated IGF2BP1-regulated stabilization of KIF3C mRNA. (A) RT-qPCR showing METTL3 expression in PCa cell lines. (B) RT-qPCR showing METTL3 knockdown expression in C4–2B and DU145 cells. (C) RIP assay was conducted to show the interaction between METTL3 and KIF3C in PCa cells. (D) m6A RIP assay was conducted to show the m6A modification on KIF3C mRNA in C4–2B and DU145 cells. (E) RT qPCR was conducted to show the stability of KIF3C mRNA after adding ActD. (F) RIP assay was conducted to show the effects of the down-regulation of METTL3 on the interaction between IGF2BP1 and KIF3C in C4–2B and DU145 cells. (G) RT-qPCR showing the stability of KIF3C mRNA after adding ActD in C4–2B and DU145 cells. (H, I) RT-qPCR and western blot were conducted to confirm the KIF3C expression under METTL3 or IGF2BP1 silencing. Data are reported as means ± standard deviation of three independent experiments. *p < 0.05; **p < 0.01.