Research Paper Volume 13, Issue 18 pp 22332—22344

METTL3-mediated m6A modification of KIF3C-mRNA promotes prostate cancer progression and is negatively regulated by miR-320d

MiR-320d inhibited PC progression through METTL3/KIF3C. C4–2B and DU145 cells were transfected with NC mimic, miR-320d mimic, miR-320d mimic+pcDNA3.1, miR-320d mimic+pcDNA3.1/METTL3, or miR-320d mimic + pcDNA3.1/KIF3C, respectively. (A) RT-qPCR showing KIF3C expression in indicated group PCa cells. (B, C) CCK-8 and colony formation were conducted to show the proliferation of PCa cells. (D) Transwell showing invasion and migration ability of PCa cells. Data are reported as means ± standard deviation of three independent experiments. *p

Figure 5. MiR-320d inhibited PC progression through METTL3/KIF3C. C4–2B and DU145 cells were transfected with NC mimic, miR-320d mimic, miR-320d mimic+pcDNA3.1, miR-320d mimic+pcDNA3.1/METTL3, or miR-320d mimic + pcDNA3.1/KIF3C, respectively. (A) RT-qPCR showing KIF3C expression in indicated group PCa cells. (B, C) CCK-8 and colony formation were conducted to show the proliferation of PCa cells. (D) Transwell showing invasion and migration ability of PCa cells. Data are reported as means ± standard deviation of three independent experiments. *p < 0.05; **p < 0.01.