Figure 5. SKP2, GSTM2, and ASPA silencing did not affect the level of sh-SUV39H2-induced cardiomyocyte senescence. H9C2 cells were pretreated with 5% DMEM for 8 h, and then transfected with interfering RNA and control si-NC for 48 h. Transfection efficiency was analyzed and quantified by western blotting and RT-PCR. There are three siRNA sequence: SKP2 (A, D), GSTM2 (B, E), GSTM2 (C, F). The blue region shows senescence-positive cells, and the proportion of positive cells in three random fields was quantified. No differences were observed across experiment groups (G). Data are expressed as the mean ± standard deviation. All the experiments have been repeated independently at least 3 times. *P < 0.05, **P < 0.01, ***P < 0.005 when two groups were compared as indicated, or compared to the corresponding control.