Research Paper Volume 13, Issue 18 pp 21975—21990

Thymus hirtus sp. algeriensis Boiss. and Reut. volatile oil enhances TRAIL/Apo2L induced apoptosis and inhibits colon carcinogenesis through upregulation of death receptor pathway

(A) TS downregulates (Ai) antiapoptotic, (Aii, Aiii) proliferative, and metastatic gene products. Briefly, colon cancer cells were treated with indicated stimuli for 24 h. Whole cell protein extracts were prepared, separated by electrophoresis, and then transferred to the nitrocellulose membrane using the antibodies indicated and relative protein expression levels were quantified. (B) TS sensitizes TRAIL-induced change in antiapoptotic, proliferative and metastatic gene product levels. HCT116 cells (1 × 106 cells/well) treated with either thyme volatile oil and TRAIL for the indicated times (6 h and 24 h). Whole-cell lysates were subjected to Western blotting analysis using relevant antibodies and relative protein expression levels were quantified. The same blots were stripped and reprobed with β-actin antibodies to verify equal protein loading. These are representative results of three independent experiments. * P˂ 0.05, ** P ˂ 0.01 compared with control group. § P ˂ 0.05 compared with TS group.

Figure 5. (A) TS downregulates (Ai) antiapoptotic, (Aii, Aiii) proliferative, and metastatic gene products. Briefly, colon cancer cells were treated with indicated stimuli for 24 h. Whole cell protein extracts were prepared, separated by electrophoresis, and then transferred to the nitrocellulose membrane using the antibodies indicated and relative protein expression levels were quantified. (B) TS sensitizes TRAIL-induced change in antiapoptotic, proliferative and metastatic gene product levels. HCT116 cells (1 × 106 cells/well) treated with either thyme volatile oil and TRAIL for the indicated times (6 h and 24 h). Whole-cell lysates were subjected to Western blotting analysis using relevant antibodies and relative protein expression levels were quantified. The same blots were stripped and reprobed with β-actin antibodies to verify equal protein loading. These are representative results of three independent experiments. * P˂ 0.05, ** P ˂ 0.01 compared with control group. § P ˂ 0.05 compared with TS group.