Research Paper Volume 13, Issue 18 pp 22459—22473

miR-19b-3p relieves intervertebral disc degeneration through modulating PTEN/PI3K/Akt/mTOR signaling pathway

miR-19b-3p expression was impeded in IL-1β/TNF-α/hydrogen peroxide-treated HNPCs. HNPCs were treated with IL-1β (10 ng/mL), TNF-α (40 ng/mL) and hydrogen peroxide (500 μM) for 24 hours to establish an in vitro IVDD model. (A) TUNEL staining was employed for apoptosis detection. (B) The miR-19b-3p level was testified by qRT-PCR. (C, D) The profiles of matrix metalloproteinases MMP-3, MMP-9, MMP-13, ADAMTS-4 and ADAMTS-5 were compared by qRT-PCR and WB. (E) The PTEN/PI3K/Akt/mTOR profile was verified by WB. * P P P

Figure 2. miR-19b-3p expression was impeded in IL-1β/TNF-α/hydrogen peroxide-treated HNPCs. HNPCs were treated with IL-1β (10 ng/mL), TNF-α (40 ng/mL) and hydrogen peroxide (500 μM) for 24 hours to establish an in vitro IVDD model. (A) TUNEL staining was employed for apoptosis detection. (B) The miR-19b-3p level was testified by qRT-PCR. (C, D) The profiles of matrix metalloproteinases MMP-3, MMP-9, MMP-13, ADAMTS-4 and ADAMTS-5 were compared by qRT-PCR and WB. (E) The PTEN/PI3K/Akt/mTOR profile was verified by WB. * P <0.05, ** P <0.01, *** P <0.001(vs. control group). N=3.