Research Paper Volume 13, Issue 18 pp 22459—22473

miR-19b-3p relieves intervertebral disc degeneration through modulating PTEN/PI3K/Akt/mTOR signaling pathway

Overexpressing miR-19b-3p attenuated the inhibition of PTEN overexpression on HNPCs. HNPCs were transfected with PTEN overexpression plasmids or miR-19b-3p mimics. (A, B) The miR-19b-3p profile was checked by qRT-PCR. (C) WB was performed to compare the levels of MMP-3, MMP-9, MMP-13, ADAMTS-4 and ADAMTS- in HNPCs. (D) The PTEN/PI3K/Akt/mTOR expression tested by WB. (E) Cell proliferation was gauged by CCK-8. (F) TUNEL staining was performed to assess cell apoptosis. * P P P P P

Figure 7. Overexpressing miR-19b-3p attenuated the inhibition of PTEN overexpression on HNPCs. HNPCs were transfected with PTEN overexpression plasmids or miR-19b-3p mimics. (A, B) The miR-19b-3p profile was checked by qRT-PCR. (C) WB was performed to compare the levels of MMP-3, MMP-9, MMP-13, ADAMTS-4 and ADAMTS- in HNPCs. (D) The PTEN/PI3K/Akt/mTOR expression tested by WB. (E) Cell proliferation was gauged by CCK-8. (F) TUNEL staining was performed to assess cell apoptosis. * P <0.05, ** P <0.01, *** P <0.001(vs. Vector group). & P <0.05,&& P <0.01(vs. Vector+miR-NC group). N=3.