Figure 2. Norbixin does not transactivate PPARs, but inhibits PPARγ transactivation induced by TGZ. Effect of GW9578, GW0742 and TGZ and of increasing norbixin (NBX) concentrations on over-expressed PPARα (A), PPARβ/δ (B) and PPARγ (C). Effect of GW9578 (20 μM), of GW0742 (30 μM) and of TGZ (20 μM) alone or in competition with NBX (20 μM) on over-expressed PPARα (D), PPARβ/δ (E) and PPARγ (F) transactivation. Bars represent mean ± s.e.m. with n = 3–6. *p < 0.05, **p < 0.01, ****p < 0.0001 compared to CONT corresponding to DMSO alone added to the medium; #p < 0.01 compared to TGZ (One-way ANOVA, Dunnett’s post-test).