Research Paper Volume 13, Issue 18 pp 22490—22501

Nesfatin-1 facilitates IL-1β production in osteoarthritis synovial fibroblasts by suppressing miR-204-5p synthesis through the AP-1 and NF-κB pathways

Nesfatin-1 enhances IL-1β production in human OASFs. OASFs were incubated with nesfatin-1 (0.1–1 ng/mL) and IL-1β mRNA and protein expression was examined by qPCR (A) and Western blot (B). Quantitative data for Western blot (C) and ELISA (D) assays. *p

Figure 2. Nesfatin-1 enhances IL-1β production in human OASFs. OASFs were incubated with nesfatin-1 (0.1–1 ng/mL) and IL-1β mRNA and protein expression was examined by qPCR (A) and Western blot (B). Quantitative data for Western blot (C) and ELISA (D) assays. *p < 0.05 compared with the control group.